In our research, we are using an ELISA test in order to observe IgA levels in college students while they study. Since IgA can be collected from saliva, we will ask consenting students to spit in a vile upon entering the library. Three experimental groups will be used: early morning studiers (8:00am – 10:00am), mid-day studiers (2:00pm – 4:00pm) and late night studiers (10:00pm – 12:00am). Due to the nature of IgA and its production during stress, we can see how varied times of study effect the stress levels of college students. It is also important to take into consideration the fluctuations of stress markers, such as corticosterone and IgA levels of humans during everyday life. These stress markers are released through the endocrine system and provoke emotions such as anxiety and stress. Although the varied levels of these corticosterone will not be tested, IgA levels will provide us with enough insight on the varied levels of stress of these students. We expect to observe how these stress markers are secreted during a student’s everyday life; but hope we can pinpoint a particular time period in which students are producing more IgA while studying. This study will enable us to conclude the time of day in which students whom are studying are more stressed out and provide insight on how stress markers vary dependent on the time of day.