After saliva samples were collected from students studying in the library, the samples were placed in a 96-well ELISA plate in order to be analyzed. A washing technique was conducted in order to prepare the samples in the well such that maximum binding to IgA would occur. The plate was then placed into a Photospectrometer which is basically a machine that emits light at a certain wavelength and analyzes the amount of light that is allowed through the bottom of the well. The higher the number indicates the presence of IgA bound in the well.
Raw data collected from the ELISA plate after being ran through the Photospectrometer. Numbers indicate the amount of light at a wavelength of 450nm that was allowed through the wells. Higher numbers indicate less light was able to emit through, suggesting that presence of IgA is higher when less light was able to pass through.
Raw data collected from the ELISA plate after being ran through the Photospectrometer. Numbers indicate the amount of light at a wavelength of 450nm that was allowed through the wells. Higher numbers indicate less light was able to emit through, suggesting that presence of IgA is higher when less light was able to pass through.